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KMID : 0368420070500010044
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Journal of Plant Biology
2007 Volume.50 No. 1 p.44 ~ p.44
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Transgenic tobacco expressing a ring domain-containing protein ofCapsicum annuum confers improved cold tolerance
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Kim Kyung-A
Hwang Eul-Won
Park Soo-Chul
Jeong Mi-Jeong
Byun Myung-Ok
Kwon Hawk-Bin
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Abstract
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Zinc finger proteins function in plant tolerances to stresses from cold, dehydration, and salt. To determine the mechanisms for those underlying defenses, we previously used cDNA microarrays and northern blot analysis to identify a gene for the ring zinc finger protein (RDCP1) from hot pepper (Capsicum annuum). In that study, we showed that theRDCP1 gene was strongly induced by cold stress and, to a lesser degree, by ABA and high salt Here, we have used a Ti-plasmid andAgrobacterium- mediated transformation to engineerRDCP1 under the control of the CaMV35S promoter for constitutive expression in tobacco. The resultant RDCP1 transgenic plants exhibit significantly increased tolerance to low temperatures. Moreover, some of those transgenics have greater drought tolerance. In addition, none of the RDCP1 transgenic plants show any visible alterations from the wild phenotype. These current results demonstrate the biological role of RDCP1 in conferring stress tolerance.
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KEYWORD
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Capsicum annuum, cold tolerance, ring domain-containing protein, transgenic tobacco
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